The CleanPlex® Respiratory Virus Research Panel v2 combines SARS-CoV-2 whole genome amplification with 149 strategically designed primers to cover characteristic regions that span multiple gene segments for each Influenza A subtype H1N1, H1N2, H3N2, Influenza B, and respiratory syncytial virus (RSV) types A and B. The increased target regions yield a higher coverage, resulting in higher sensitivity and making this panel more suitable for high-throughput screening strategies such as testing multi-sample pools, than qPCR methods with only 1 to 2 targets per subtype. The respiratory virus primers were expertly designed in consideration of over 45,000 viral genome sequences to generate highly specific, but also universally conserved targets for each influenza and RSV subtype to allow confident detection throughout flu seasons, regardless of changes in strain prevalence.
Built upon the CleanPlex SARS-CoV-2 Research and Surveillance Panel, the Respiratory Virus Research Panel v2 addresses the need to assay the SARS-CoV-2 and other respiratory viruses concurrently during the overlapping flu season and the COVID-19 pandemic. The panel utilizes the CleanPlex multiplex PCR-based targeted sequencing technology to provide an easy-to-use, fast, and comprehensive solution for detection and differentiation of these common viruses, influenza and RSV subtyping, and SARS-CoV-2 mutation analysis, tracking, and surveillance for informed infection control through comprehensive sequence information.
- Significant Cost Savings
99% reduction in sequencing cost. Only 30K–50K reads per sample required v.s. 10M reads required by many metagenomics sequencing methods.
- High Coverage of Target Regions
Whole-genome coverage of the SARS-CoV-2 genome and selective coverage of influenza and RSV gene segments for sub-typing
- Ultra-Sensitive Detection
Multiplex PCR based method for more sensitive detection of low viral copies.
- Fast, Streamlined Workflow
Generate sequencing-ready libraries in just 6 hours using a rapid, four-step protocol including RT step with minimal hands-on time.
- Superb Performance
Prepare high-quality NGS libraries with excellent on-target performance using CleanPlex Technology to enable efficient use of sequencing reads. CleanPlex panels’ on-target rates are usually much higher than those of hybrid captured-based small target enrichment panels.
The CleanPlex Respiratory Virus Research Panel contains the CleanPlex Multiplex PCR Primers and CleanPlex Targeted Library Kit with RT components. CleanPlex Indexed PCR Primers for sample pooling and CleanMag® Magnetic Beads for bead purification and size selection are ordered separately to complete the workflow from input RNA to sequencing-ready NGS libraries.
For a complete solution, please use the following indexed PCR primers and magnetic beads SKUs.earl
|Pack Size (Reactions)||SKUs for Indexed PCR Primers||SKUs for Magnetic Beads|
|96||1 x SKU#716006 – CleanPlex® Dual-Indexed PCR Primers for Illumina® Set A (12 x 8 indexes, 96 reactions)||1x SKU#718005 – CleanMag Magnetic Beads (20 mL, ~100 reactions for CleanPlex 2-pool panels)|
|384||1 x SKU#716037-716040- CleanPlex® Plated Unique Dual-Indexed PCR Primers for Illumina®||1 x SKU#718003 – CleanMag Magnetic Beads (60 mL, ~300 reactions for CleanPlex 2-pool panels)|
Store at -20 °C.
For Research Use Only. Not for use in diagnostic procedures.