OmniFusion™ RNA Lung Cancer Panel

OmniFusion™ RNA Lung Cancer Panel uses single primer amplification method to enables detection more than 530 known and unknown fusions associated with non-small cell lung cancer. 

 

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Product Description

OmniFusion™ RNA Lung Cancer Panel known and novel fusion detection and research via amplicon-based target enrichment for Next Generation Sequencing (NGS) on Illumina sequencing platforms

OmniFusion RNA Lung Cancer Panel for next generation sequencing enables detection more than 530 known and unknown fusions associated with non-small cell lung cancer. In contrast to the AccuFusion™ panels, OmniFusion workflow allows detection of novel fusion partners and and splicing variants. Compared to other methods such as qPCR, FISH, or Sanger sequencing, Paragon Genomics targeted fusion sequencing method allows robust multiplexed detection of variants using minimum sample input and a simple workflow (Figure 1).

OmniFusion Single-Primer Gene Fusion Detection Workflow
Figure 1. OmniFusion Single-Primer Gene Fusion Detection Workflow

The OmniFusion single-primer amplification technology uses template switching technology to add an universal sequence to the 5’ ends of RNA fragments. Combined with expertly designed targeted 3’ primers specific to acceptor genes, the process eliminates the constraint of template length, and allows higher sensitivity even with damaged RNA fragments, such as FFPE RNA. The single sided amplification method enables the detection of any mutations on the RNA fragments, including novel ones.

Learn more about the technology on our Fusion Technology page.

Highlights

  • Identification of known and novel RNA fusions associated with lung cancer
    Interrogate >530 known RNA fusion and additional novel RNA fusion using a single-primer method.
  • Fast, streamlined workflow
    Generate sequencing-ready libraries in just 6.5 hours using a rapid, five-step protocol from Extracted RNA to sequence ready libraries.
  • Excellent performance with extremely low rRNA rate
    Prepare high-quality targeted NGS libraries using OmniFusion RNA Fusion Detection Technology to achieve minimal rRNA and over 90% on-target rate.
  • Sensitive Detection
    Can detect down to 1% variant frequency with high confidence.

Storage Temperature

Store at -20 °C.

For Research Use Only. Not for use in diagnostic procedures.

ParameterSpecification
Enrichment MethodMultiplex PCR with single target specific primer
Sequencing PlatformsIllumina®
Number of Fusion Genes11 cancer driver genes ( ALK, CIT, MBIP, MET, NRG1, NTRK1, NTRK3, PDGFRA, RET, ROS1, TACC3)

3 control genes (B2M, GUSA, TBP)

Targets>530 known fusions associated with NSCLC & novel fusions
Variant TypesFusion down to 1% allele frequency
Number of Amplicons61
Amplicon Size200-700 bp (variable based on sample fragment size)
Number of Primer Pools1
Input RNA Requirement25-100 ng
Sample TypesFFPE, FNA, Fragmented RNA
Total Assay Time6.5 hours
Hands-On Time85 minutes
rRNA rate< 1%
On-Target Aligned Reads~93%

Additional Information

Weight40 g
Pack Size (Reactions)

8, 96, 384

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