AgriType® Auto Targeted Library Kit, 1-Pool

The AgriType® Auto Targeted Genotyping by Sequencing Solution is designed and developed to address large-scale agrigenomics projects. The AgriType solution is powered by our advanced primer design algorithm and an efficient ultra-high multiplex PCR-based target enrichment chemistry. AgriType products provide a cost-effective solution for constructing targeted NGS libraries to quickly and efficiently screen hundreds to thousands of markers of interest, thereby accelerating genotyping by sequencing (GBS) studies in fields such as aquaculture, livestock farming, and seed breeding.

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Product Description

The AgriType® Auto Targeted Genotyping by Sequencing Solution is designed and developed to address large-scale agrigenomics projects. The AgriType solution is powered by our advanced primer design algorithm and an efficient ultra-high multiplex PCR-based target enrichment chemistry. AgriType products provide a cost-effective solution for constructing targeted NGS libraries to quickly and efficiently screen hundreds to thousands of markers of interest, thereby accelerating genotyping by sequencing (GBS) studies in fields such as aquaculture, livestock farming, and seed breeding.

Relevant molecular breeding applications include but are not limited to Marker assisted selection (MAS), Genomic selection (GS), Quantitative trait locus (QTL) screening, Marker assisted back crossing (MABC), and Trait mapping.

Please note that this product only contains the targeted library preparation reagents without any panel specific primers. For a complete target enrichment kit, please submit your custom panel design request via our ParagonDesigner portal so that we can synthesize the primers for your GBS projects.  

Highlights

• High-Throughput Genotyping Assay 96-well and 384-well plate compatible

• Streamlined, Automation-Friendly Workflow Simple, three-step workflow with less than 25 min hands-on time that enables operators to walk away with easy automation integration

• Scalable, Adaptable Genotyping System Target up to 7,000 relevant amplicons per pool

• Cost-Effective Sequencing on Major Platforms Process thousands of samples at a time on either Illumina®, MGI DNBSeq, or Ion Torrent™ NGS platforms

• High-Quality, Reproducible Results Prepare high-quality NGS libraries with accurate call rates, excellent uniformity and on-target rates for efficient use of sequencing reads

High-Quality AgriType NGS Panels for All Panel Sizes AgriType panels show consistently robust sequencing metrics across various crop types and panel sizes for cost-effective sequencing. Using extracted DNA from leaf and seed samples from distinct crops, libraries were prepared and sequenced at 100X coverage on an Illumina MiSeq. The table below shows uniformity, on-target, and mean call rates for selected crops.

As shown in the BioAnalyzer trace below, the negligible background noise across different sample types attest to high-quality and clean sequencing performance.

Additional Information

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CleanPlex Amplicon Sequencing Library Kit Product ImageAgriType® Auto Targeted Library Kit, 1-Pool