CleanPlex SARS-CoV-2 FLEX Kit for COVID-19 coronavirus detection, tracking, and research via amplicon-based target enrichment for Next Generation Sequencing (NGS) on Illumina sequencing platforms
Paragon Genomics designed a highly multiplexed target enrichment panel covering the entire genome of SARS-CoV-2 virus (except for 92 bases at the ends). Our original SARS-CoV-2 Research and Surveillance Kit enables complete genome sequencing and epidemiological studies of the new SARS-CoV-2 virus responsible for the COVID-19 pandemic.
SARS-COV-2 FLEX Kit differs from the standard SARS-COV-2 Kit in that it contains degenerate primer designs of polymorphic regions of the genome to allow more robust amplification of variable strains. The FLEX panel also contains a human positive control that amplifies at 100bp longer than the viral library peak to serve as an internal library preparation control, especially for negative samples. CleanPlex technology allows the ease and flexibility to sequence samples for confident infectious disease surveillance and research.
To ensure that our customers get the most out of their sequencing runs, we’re proud to offer up to 2,688 sample multiplexing capability with our combinatorial dual-indexed primers for Illumina sequencing. Additionally, we can provide 384 unique-dual indexes, allowing for low variant calling and other in-depth sequencing applications. These exciting additions allow for cost-effective and high-throughput sequencing with our best-in-class CleanPlex targeted sequencing technologies.
With our CleanPlex technology, the entire genome of the virus can be amplified from RNA to sequence-ready libraries in 5.5 hours and our panel has repeatedly demonstrated consistently high coverage of multiple variants. Read more about how the panel has been used around the globe for surveillance and research.
Highlights of the FLEX panel
- Degenerate Primers
Strategically designed for consistent coverage to withstand mutations over time.
- Improved Coverage
Optimized amplification of specific regions in the genome for more uniform coverage even at low input amount.
- Fast, Streamlined Workflow
Generate libraries for Illumina® platforms in just 5.5 hours using a simple, four-step protocol
- In-Process Controls
Human RNA primers are included as library preparation controls for more confident negative calls.
- Degenerate Primers
The CleanPlex SARS-CoV-2 Kit contains the CleanPlex Multiplex PCR Primers and CleanPlex Targeted Library Kit with RT components. CleanPlex Indexed PCR Primers for sample pooling and CleanMag® Magnetic Beads for bead purification and size selection are ordered separately to complete the workflow from input RNA to sequencing-ready NGS libraries.
For a complete solution, please use the following indexed PCR primers and magnetic beads SKUs.
|Pack Size (Reactions)||SKUs for Indexed PCR Primers||SKUs for Magnetic Beads|
|96||1 x SKU#716006 – CleanPlex® Dual-Indexed PCR Primers for Illumina® Set A (12 x 8 indexes, 96 reactions)||1 x SKU#718005 – CleanMag Magnetic Beads (20 mL, ~100 reactions for CleanPlex 2-pool panels)|
|384||1 x SKU#716017 – CleanPlex® Dual-Indexed PCR Primers for Illumina® Set A (12 x 8 indexes, 384 reactions)||1 x SKU#718003 – CleanMag Magnetic Beads (60 mL, ~300 reactions for CleanPlex 2-pool panels)|
|4 X 96||1 x Each of SKU#716037 to 716040 – CleanPlex® Unique Dual-Indexed PCR Primers for Illumina® Set C, D, E, F 96-well Plated (96 reactions)|
*Note: Use 8 sets of 384 on NovaSeq dual S4 flow cell sequencing for maximum 3072 sample multiplexing.
|1 x SKU#718003 – CleanMag Magnetic Beads (60 mL, ~300 reactions for CleanPlex 2-pool panels)|
Store at -20 °C.
For Research Use Only. Not for use in diagnostic procedures.