CleanPlex® SARS-CoV-2 FLEX Panel

This amplicon-based SARS-CoV-2 FLEX NGS panel is designed for Coronavirus research and surveillance, enabling complete genome sequencing of the new SARS-CoV-2 virus responsible for the COVID-19 pandemic. The FLEX panel is built upon the original SARS-CoV-2 Panel and contains additional components for more robust and confident variant calling even when the virus mutates over time. 

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Product Description

CleanPlex® SARS-CoV-2 FLEX Panel for COVID-19 coronavirus detection, tracking, and research via amplicon-based target enrichment for Next Generation Sequencing (NGS) on Illumina sequencing platforms

Paragon Genomics designed a highly multiplexed target enrichment panel covering the entire genome of SARS-CoV-2 virus (except for 92 bases at the ends). The SARS-CoV-2 Research and Surveillance panel enables complete genome sequencing and epidemiological studies of the new SARS-CoV-2 virus responsible for the COVID-19 pandemic. With Paragon Genomics CleanPlex technology, the entire genome of the virus can be amplified from RNA to sequence-ready libraries in 5.5 hours. 

SARS-COV-2 FLEX panel differs from the standard SARS-COV-2 Panel in that it contains degenerate primer designs of polymorphic regions of the genome to allow more robust amplification of variable strains. The FLEX panel also contains a human positive control that amplifies at 100bp longer than the viral library peak to serve as an internal library preparation control, especially for negative samples. CleanPlex technology allows the ease and flexibility to sequence samples for confident infectious disease surveillance and research.

To ensure that our customers get the most out of their sequencing runs, we’re proud to offer up to 2,688 sample multiplexing capability with our combinatorial dual-indexed primers for Illumina sequencing. Additionally, we can provide 384 unique-dual indexes, allowing for low variant calling and other in-depth sequencing applications. These exciting additions allow for cost-effective and high-throughput sequencing with our best-in-class CleanPlex targeted sequencing technologies.

Highlights of the FLEX panel

    • Degenerate Primers
      Strategically designed for consistent coverage to withstand mutations over time.
    • Improved Coverage
      Optimized amplification of specific regions in the genome for more uniform coverage even at low input amount.
    • Fast, Streamlined Workflow
      Generate libraries for Illumina® platforms in just 5.5 hours using a simple, four-step protocol
    • In Process Controls
      Human RNA primers are included as library preparation controls for more confident negative calls.

The CleanPlex SARS-CoV-2 Panel contains the CleanPlex Multiplex PCR Primers and CleanPlex Targeted Library Kit with RT components. CleanPlex Indexed PCR Primers for sample pooling and CleanMag® Magnetic Beads for bead purification and size selection are ordered separately to complete the workflow from input RNA to sequencing-ready NGS libraries.

For a complete solution, please use the following indexed PCR primers and magnetic beads SKUs.

Pack Size (Reactions)SKUs for Indexed PCR PrimersSKUs for Magnetic Beads
961 x SKU#716006 – CleanPlex® Dual-Indexed PCR Primers for Illumina® Set A (12 x 8 indexes, 96 reactions)1 x SKU#718002 – CleanMag Magnetic Beads (20 mL, ~100 reactions for CleanPlex 2-pool panels)
3841 x SKU#716017 – CleanPlex® Dual-Indexed PCR Primers for Illumina® Set A (12 x 8 indexes, 384 reactions)1 x SKU#718003 – CleanMag Magnetic Beads (60 mL, ~300 reactions for CleanPlex 2-pool panels)

1 x SKU#718002 – CleanMag Magnetic Beads (20 mL, ~100 reactions for CleanPlex 2-pool panels)

4 X 961 x Each of SKU#716037 to 716040  – CleanPlex® Unique Dual-Indexed PCR Primers for Illumina® Set C, D, E, F 96-well Plated (96 reactions)

*Note: Use 8 sets of 384 on NovaSeq dual S4 flow cell sequencing for maximum 3072 sample multiplexing.

1 x SKU#718003 – CleanMag Magnetic Beads (60 mL, ~300 reactions for CleanPlex 2-pool panels)

1 x SKU#718002 – CleanMag Magnetic Beads (20 mL, ~100 reactions for CleanPlex 2-pool panels)

Storage Temperature

Store at -20 °C.

For Research Use Only. Not for use in diagnostic procedures.

Additional Information

WeightN/A
Pack Size (Reactions)

8, 96, 384

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For early access to our upcoming Respiratory Research Panel combining Influenza A/B and SARS-CoV-2 testing, please visit HERE. Dismiss