Product Description
The CleanPlex Plated Dual-Indexed PCR Primers for Illumina® are high-quality ready-to-use PCR primers for Illumina library construction. They are compatible and designed for use with all CleanPlex Panels to construct targeted libraries for sequencing on an Illumina NGS platform. The index set comprises of i5 primers and i7 primers that form combinatorial indexing of samples for multiplexing samples per sequencing run. Samples with unique i5 and i7 combinations can be pooled and sequenced then demultiplexed for analysis.
| CleanPlex Plated Dual-Indexed PCR Primers for Illumina | ||||
| Description | SKU | Reactions | Available combinations/Kit | Format |
| CleanPlex Dual-Indexed PCR Primers for Illumina Set T (3 x 3 indexes, 9 reactions) | 716021 | 9 | 3 x 3, 9 combinations | 6 Tubes |
| CleanPlex® Plated Dual-Indexed PCR Primers for Illumina® Set A (96 reactions) | 716033 | 96 | 8 x 12 , 96 index combinations | 96 well plate |
| CleanPlex® Plated Dual-Indexed PCR Primers for Illumina® Set B (96 reactions) | 716034 | 96 | 8 x 12 , 96 index combinations | 96 well plate |
| CleanPlex® Plated Dual-Indexed PCR Primers for Illumina® Set C (96 reactions) | 716035 | 96 | 8 x 12 , 96 index combinations | 96 well plate |
| CleanPlex® Plated Dual-Indexed PCR Primers for Illumina® Set D (96 reactions) | 716036 | 96 | 8 x 12 , 96 index combinations | 96 well plate |
| CleanPlex® Plated Dual-Indexed PCR Primers for Illumina® Set A (384 reactions) | 716045 | 384 | 8 x 12 , 96 index combinations, 4 rxn each well | 96 well plate |
| CleanPlex® Plated Dual-Indexed PCR Primers for Illumina® Set B (384 reactions) | 716046 | 384 | 8 x 12 , 96 index combinations, 4 rxn each well | 96 well plate |
| CleanPlex® Plated Dual-Indexed PCR Primers for Illumina® Set C (384 reactions) | 716047 | 384 | 8 x 12 , 96 index combinations, 4 rxn each well | 96 well plate |
| CleanPlex® Plated Dual-Indexed PCR Primers for Illumina® Set D (384 reactions) | 716048 | 384 | 8 x 12 , 96 index combinations, 4 rxn each well | 96 well plate |
*Sets A & B index sequences are the same index sequences that was provided in the (discontinued) tubed format. Set C and D are cross combined i5 and i7 indexes from Set A and Set B sequences to form the full 384 combinatorial index pairs.
Primer Sequences:
i5 Indexed Primers
5’-AATGATACGGCGACCACCGAGATCTACACXXXXXXXXACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′
i7 Indexed Primers
5’-CAAGCAGAAGACGGCATACGAGATXXXXXXXXGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3’
Notes:
- Please refer to CleanPlex NGS Panel User Guide for detailed instructions on using CleanPlex Dual-Indexed PCR Primers for Illumina
- Set A and B plated Dual-Indexed primers share the same index sequences as the (discontinued) Set A and B tubed format indexes
- Please contact sales for bulk options of more than 384 reactions per plate.
Storage Temperature:
Store at -20°C
For Research Use Only. Not for use in diagnostic procedures.
