CleanPlex® ACE2 & TMPRSS2 Panel

The CleanPlex® ACE2 & TMPRSS2 Panel is a multiplex PCR-based targeted sequencing assay designed to examine the two genes associated with COVID-19 susceptibility. Confidently detect mutations related to SARS-COV-2 virus S protein and ACE2 binding affinity and expression of ACE2 and TMPRSS2. 

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Product Description

The CleanPlex® ACE2 & TMPRSS2 Panel is a multiplex PCR-based targeted sequencing assay designed to examine two genes associated with COVID-19 susceptibility. 

Research has shown that the SARS-CoV-2 virus is internalized by the binding of its S protein to the host ACE2 with the help of TMPRSS2. The binding affinity of ACE2, along with ACE2 and TMPRSS2 expression levels, are major determinants of SARS-CoV-2 replication rate and disease severity. This panel targets all exonic and promoter regions of the two genes, and more than 70 expertly curated expression quantitative trait loci (eQTL), shown to regulate gene expression.

The CleanPlex ACE2 & TMPRSS2 panel facilitates further investigations in host genome mutations related to the ACE2 binding affinity and expression of ACE2 and TMPRSS2, which could provide additional insights into an individual’s viral susceptibility and their disease course.

Highlights

  • Curated Content and Comprehensive Data
    Interrogates entire ACE2 & TMPRSS2 coding and promotor regions plus curated 70+ eQTL targets
  • Fast, Streamlined Workflow
    Generate libraries for Illumina® platforms in just 3 hours using a simple, three-step protocol
  • Superb Performance with Low DNA Input
    Prepare high-quality NGS libraries with excellent coverage uniformity and on-target performance to enable efficient use of sequencing reads and reduce costs

The CleanPlex ACE2/TMPRSS2 Panel contains CleanPlex Multiplex PCR Primers and CleanPlex Targeted Library Kit. CleanPlex Dual-Indexed PCR Primers, CleanPlex Unique Dual-Indexed PCR Primers and CleanMag® Magnetic Beads are ordered separately to complete the workflow from input DNA to sequencing-ready NGS libraries.

Storage Temperature

Store at -20 °C.

For Research Use Only. Not for use in diagnostic procedures.

Additional Information

Weight40 g
Dimensions10 x 7 x 5 cm
Pack Size (Reactions)

8, 96

Accurate Variant Calls

Three cell lines were sequenced and all expected mutations were called. The table below also shows a subset of calls made in NA12878 that includes point mutations and indels. Variant analysis shows that the CleanPlex ACE2 & TMPRSS2 Panel provides accurate and confident calling for expected homozygous and heterozygous germline mutations.

ACE2 TMPRSS2 concordance data
ACE2 TMPRSS2 concordance data

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For early access to our upcoming Respiratory Research Panel combining Influenza A/B and SARS-CoV-2 testing, please visit HERE. Dismiss