CleanPlex® Unique Dual-Indexed PCR Primers for Illumina®

$288.00$624.00

The CleanPlex® Unique Dual-Indexed PCR Primers for Illumina® are high-quality ready-to-use PCR primers for Illumina library construction. They are compatible and designed for use with all CleanPlex and CleanPlex UMI NGS Panels to constructed targeted libraries for sequencing on an Illumina NGS platform.

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Product Description

The CleanPlex® Unique Dual-Indexed (UDI) PCR Primers for Illumina® are high-quality ready-to-use PCR primers for Illumina library construction. They are compatible and designed for use with all CleanPlex Panels to construct targeted libraries for sequencing on an Illumina NGS platform. The index set comprises of uniquely pooled i5 and i7 index primer pools for unique dual multiplexing of samples per sequencing run. Each set of UDI primer pool only contains one instance of each i5 and each i7 to mitigate index hopping concerned on Illumina sequencing platforms with patterned flowcells. Samples indexes with each i5 and i7 pair can be pooled and sequenced then demultiplexed for analysis. 

CleanPlex Unique Dual-Indexed (UDI) PCR Primers for Illumina 
DescriptionSKUReactionsAvailable Unique Indexes/KitFormat
CleanPlex® Unique Dual-Indexed PCR Primers for Illumina Set T8
(8 indexes, 8 reactions)
716015
(Available upon request)
8 8 Unique Index Pairs, (Random selection of Set A or Set B indexes)8 Tubes
CleanPlex® Unique Dual-Indexed PCR Primers for Illumina Set A *
(16 indexes, 32 reactions)
71601132 16 Unique Index pairs16 Tubes
CleanPlex® Unique Dual-Indexed PCR Primers for Illumina Set A
(16 indexes, 96 reactions)
71601296 16 Unique Index pairs16 Tubes
CleanPlex® Unique Dual-Indexed PCR Primers for Illumina Set B*
(16 indexes, 32 reactions)
71601332 16 Unique Index pairs16 Tubes
CleanPlex® Unique Dual-Indexed PCR Primers for Illumina Set B
(16 indexes, 96 reactions)
71601496 16 Unique Index pairs16 Tubes

*Sets A and B’s index sequences are mutually exclusive. Can be combined for a total of 32 available UDI options.

Primer Sequences:

i5 Indexed Primers

5’-AATGATACGGCGACCACCGAGATCTACACXXXXXXXXACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′

i7 Indexed Primers

5’-CAAGCAGAAGACGGCATACGAGATXXXXXXXXGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3’

Notes:

  • Please refer to CleanPlex UMI NGS Panel User Guide for instructions on using CleanPlex Dual-Indexed PCR Primers for Illumina
  • This product listing is for indexes delivered in single tube format, not 96 well plated format.
  • Set A and Set B index sequences are mutually exclusive and can be sequenced together for a total of 32 sample multiplexing 

Storage Temperature:

Store at -20°C

For Research Use Only. Not for use in diagnostic procedures.

Product Documents

Additional Information

Weight40 g
Reactions

Set A 32, Set A 96, Set B 32, Set B 96

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