The CleanPlex® TMB 500 Kit is a targeted resequencing assay designed to enable rapid and accurate assessment of tumor mutational burden and comprehensive cancer profiling. The kit includes an expertly curated panel using the latest research findings to target 516 genes associated with major solid tumor types and maintains >96% correlation with whole exome datasets. The panel covers 1.64 Mb of genomic content (1.19 Mb exonic) and targets both single nucleotide variants (SNVs) and insertion-deletion mutations (indels). Two pools of multiplex PCR primers are utilized to construct target-enriched libraries from either formalin-fixed paraffin-embedded (FFPE) DNA from tumor samples or high-quality gDNA from blood samples. Starting with just 20 ng of DNA (10 ng per primer pool), sequencing-ready libraries can be prepared using a streamlined workflow in just 6 hours, allowing samples to be sequenced on the same day.
- Comprehensive Content
Interrogate 516 genes associated with the major solid tumor types to assess tumor mutational burden and characterize mutation profile
- Fast, Streamlined Workflow
Generate sequencing-ready libraries in just 6 hours using a rapid, three-step protocol
- Excellent Correlation with Whole Exome Sequencing
Prepare high-quality targeted NGS libraries using CleanPlex Technology to replace the costly and time-consuming workflow of hybridization-based target enrichment
The CleanPlex TMB 500 Kit contains CleanPlex Multiplex PCR Primers and CleanPlex Targeted Library Kit. CleanPlex Indexed PCR Primers and CleanMag® Magnetic Beads are ordered separately to complete the workflow from input DNA to sequencing-ready NGS libraries.
Store at -20 °C.
For Research Use Only. Not for use in diagnostic procedures.