CleanPlex® Amplicon-Based NGS Panels for Amplicon Sequencing

I was very impressed by how well the CleanPlex SARS-CoV-2 panel worked

I was very impressed by how well the CleanPlex SARS-CoV-2 panel worked when we evaluated it back in March. Working with Paragon Genomics, we were able to leverage the panel to quickly launch a SARS-CoV-2 whole-genome sequencing assay and used it effectively for a number of genomic epidemiology studies.”

Xiaowu Gai, PhD, Director of Bioinformatics, Children's Hospital Los Angeles

A custom CleanPlex UMI panel enables the detection of low-frequency somatic mutations in CHiP genes (Clonal Hematopoiesis of Indeterminate Potential). 

Paragon Genomics has streamlined our workflow, from assay design and optimization to laboratory implementation. Their expertise helped us successfully implement a fully automated process using a custom-designed CleanPlex UMI panel, enabling low variant allele frequency (VAF) detection critical for our projects. Paragon’s excellent bioinformatics services and customer support services were key to achieving our goals.”

Ian Mongrain, Director of Operations, Montreal Heart Institute - Pharmacogenomics Centre

Affordable and easy-to-use product

When we decided to choose a targeted sequencing platform, we had several choices and I am delighted that we chose Paragon Genomics. Paragon Genomics has enabled us to move our sequencing in-house with an affordable and easy-to-use product. They assisted us every step along the way from choosing the amplicons, to guiding us through library preparation to assisting with analysis. I strongly recommend their CleanPlex^® amplicon-based targeted sequencing approach and could not be happier with their product.”

Elliot Stieglitz, MD, Associate Professor, University of California San Francisco

Complete viral genomes from clinical samples with low viral content

We’re using Paragon’s CleanPlex and CleanPlex UMI technologies to sequence RNA viruses. These technologies enable us to rapidly and cost-effectively obtain complete viral genomes from clinical samples with low viral content. Further analyses of viral genomes, including the identification of minor variants, elucidates viral diversity and evolution. Paragon has enthusiastically worked with us to tackle this new application; they have diligently designed panels to our specifications, meeting with us frequently to optimize the design.”

Aaron Lin, PhD; Erica Normandin; Kim Lagerborg, The Broad Institute of MIT and Harvard

Great PCR uniformity and on-target rate

Paragon Genomics designed a high-quality NGS custom panel for us within two weeks. Its CleanPlex^® targeted library technology is fast and easy to use with great PCR uniformity and on-target rate. We are very satisfied by CleanPlex product performance and the superb technical and customer support provided by the Company.”

Chongjian Chen, PhD, CEO, Annoroad

A ‘future proof method’ and a ‘one and done’ approach

We are looking for a ‘future proof method’, a better way to do high throughput screening at a species level diagnosis. Paragon Genomics has developed a rapid 2.5-hour library preparation allowing multi-gene methodology, letting us look at lots of unique loci across multiple genomes. It offers a ‘one and done’ approach instead of many different disconnected experiments.”

Scott Geib, PhD, Research Entomologist, USDA-ARS