CleanPlex® SARS-CoV-2 Panel for COVID-19 coronavirus detection, tracking, and research via amplicon-based target enrichment for Next Generation Sequencing (NGS) on Illumina sequencing platforms
Paragon Genomics designed a highly multiplexed target enrichment panel covering the entire genome of the SARS-CoV-2 virus (except for 92 bases at the ends). The panel enables complete genome sequencing and epidemiological studies of the new SARS-CoV-2 virus responsible for the COVID-19 pandemic. With Paragon Genomics CleanPlex technology, the entire genome of the virus can be amplified from RNA to sequence-ready libraries in 5.5 hours. Two versions of this panel are available for two major sequencing platforms: Illumina and MGI. CleanPlex Technology allows the ease and flexibility to sequence samples for confident infectious disease surveillance and research.
The SARS-CoV-2 FLEX panel is also available for front end leading coronavirus research customers and those who may benefit from internal library preparation control. Find out more about the SARS-CoV-2 FLEX panel here.
To ensure that our customers get the most out of their sequencing runs, we’re proud to offer up to 2688 sample multiplexing capability with our combinatorial dual-indexed primers for Illumina sequencing. Additionally, we provide 384 unique-dual indexes, allowing for low variant calling and other in-depth sequencing applications. These exciting additions allow for cost-effective and high-throughput sequencing with our best-in-class CleanPlex targeted sequencing technologies.
- Significant Cost Savings
99% reduction in sequencing cost. Only 50K reads per sample required v.s. 10M reads required by many shotgun metagenomics sequencing methods.
- High Coverage of Target Regions
99% coverage of the entire SARS-CoV-2 genome
- Sensitive Detection
Can detect down to 1 copy with high confidence compared 3-5 copies by RT-qPCR (1)
- Fast, Streamlined Workflow
Generate sequencing-ready libraries in just 5.5 hours using a rapid, four-step protocol including RT step with minimal hands-on time.
- Superb Performance
Prepare high-quality NGS libraries with excellent on-target performance using CleanPlex Technology to enable efficient use of sequencing reads. CleanPlex panels’ on-target rates are usually much higher than those of hybrid captured-based small target enrichment panels.
The CleanPlex SARS-CoV-2 Panel contains the CleanPlex Multiplex PCR Primers and CleanPlex Targeted Library Kit with RT components. CleanPlex Indexed PCR Primers for sample pooling and CleanMag® Magnetic Beads for bead purification and size selection are ordered separately to complete the workflow from input RNA to sequencing-ready NGS libraries.
For a complete solution, please use the following indexed PCR primers and magnetic beads SKUs.
|Pack Size (Reactions)||SKUs for Indexed PCR Primers||SKUs for Magnetic Beads|
|96||1 x SKU#716006 – CleanPlex® Dual-Indexed PCR Primers for Illumina® Set A (12 x 8 indexes, 96 reactions)||4 x SKU#718002 – CleanMag Magnetic Beads (5 mL, ~25 reactions for CleanPlex 2-pool panels)|
|384||1 x SKU#716017 – CleanPlex® Dual-Indexed PCR Primers for Illumina® Set A (12 x 8 indexes, 384 reactions)||1 x SKU#718003 – CleanMag Magnetic Beads (60 mL, ~300 reactions for CleanPlex 2-pool panels)|
Store at -20 °C.
(1) Corman VM et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988269/
For Research Use Only. Not for use in diagnostic procedures.