CRISPR QC: CRISPR Genome Editing Technology with NGS

CRISPR QC: CRISPR Genome Editing Technology with NGS

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) is a powerful genome editing technology that is transforming genetics and is associated with the system CRISPR-Cas9. Specifically, genome editing technology makes it possible to mutate, silence, and modify with regions of interest with high levels of precision.

CRISPR coupled with Next-Generation Sequencing (NGS) enables researchers to screen multiple genomic targets at once in a single tube to investigate both on- and off-target edits. As NGS can provide a high level of multiplexing, it can simultaneously analyze multiple target regions or samples, and it has the capability to provide single-base pair resolution, allowing for the detection of specific genetic changes tit the nucleotide level. This makes it a powerful tool for characterizing the genome post-CRISPR.

Applications of CRISPR Genome Editing Technology

The CRISPR-Cas9 system can be applied to drug discovery and development, animal disease modeling, genetically modified plant technology, biofuel technology, and gene therapy. For example, various research has indicated that CRISPR technology could be applied to specific diseases such as cancers, AIDS, sickle cell, muscular dystrophy, and others.

Confirming CRISPR Knock-Outs with NGS

NGS is a powerful tool for validating CRISPR-Cas9 knock-outs. The targeted genomic region is initially amplified via PCR and then sequenced using NGS. The technology reduces hands-on time for the most cost-effective and sensitive results.

Targeted NGS panels make it possible to confirm not only knock-outs but also knock-ins, edits, and potential off-target effects.

Why Choose CleanPlex NGS Sequencing Panels for Gene Editing Confirmation

Our experts at Paragon Genomics deliver custom assays in a complete kit form for effective confirmation of gene editing, based on the select targets by researchers. This CRISPR off-target analysis will enable researchers to leverage data regarding their off-target effects to refine their CRISPR/Cas9 strategies.

The CleanPlex NGS Sequencing Panels provide a quick turnaround time (less than 4 weeks from design acceptance to shipment) and high performance as a cost-effective sequencing solution.

CRISPR QC: Why Quality Control is Needed in CRISPR Experiments with NGS

Quality control is important for several reasons when it comes to CRISPR experiments, including:

Accuracy of Genetic Changes: Quality control must be established to confidently measure gene editing efficiency and reduce the chance of off-target effects occurring. Otherwise, there is the risk of incorrect results and interpretations.

Experimental Reproducibility: Without the necessary quality controls in place, CRISPR experiments would not be able to be replicated and repeated.

Safety: Quality control is important for maintaining a specific standard of safety for the subjects. This is especially true for CRISPR experiments that could be applied for therapeutic applications.