What is the difference between hybridization/capture-based and multiplex PCR-based target enrichment technologies?

What would you do with the extra time?

Next- generation sequencing (NGS) technologies have accelerated research efforts especially with multiplex PCR (mPCR). Scientists are capable of utilizing NGS to detect and analyze gene sequences in humans,animals, microorganisms, and plants for species authentication, de novo sequencing, and variant identification. Amplicon- based targeted library preparation methods, utilizing mPCR, are preferred by many as the more time and cost-efficient method over hybrid-capture methods. CleanPlex® technology’s simple workflow produces NGS libraries with low background, which means you only sequence the targets of interest and none of the non-specific by-products.

Paragon Genomics CleanPlex® System yields libraries with low background, no apparent GC bias, and >95% uniformity with as little as 1 ng of DNA. The system has a limit of detection below 1% and high mapping and on-target rates over a wide range of target numbers and input DNA amounts.

2018-05-11T12:11:12+00:00 February 1st, 2018|Videos|Comments Off on What is the difference between hybridization/capture-based and multiplex PCR-based target enrichment technologies?