Fast, single-tube workflow
Input to target-enriched NGS libraries
in a single-tube in 2.5 hours
CleanPlex® NGS panels feature a rapid, single-tube workflow that can be completed in 2.5 hours and requires only 30 min of hands-on time. High-quality target-enrich libraries can be easily and quickly prepared for faster time to results.
Single-tube workflow minimizes sample loss to preserve genomic information in the sample. Single-tube workflow also reduces the likelihood of errors and sample mix ups to ensure positive sample identification.
Simple workflow translates to high reproducibility
CleanPlex®‘s streamlined, single-tube workflow requires few steps and pipetting operations to convert input DNA to target enriched NGS libraries. The protocol requires no mechanical or enzymatic fragmentation, no lengthy and laborious hybridization procedure, and no expensive equipment. This means CleanPlex protocol can be easily trained and consistently performed between replicates, runs, and operators, ensuring high reproducibility and reliability in the data obtained.
High reproducibility. Eighteen CleanPlex® libraries were generated by 3 operators and sequenced on an Illumina platform. The GC coverage of the libraries were plotted and compared against each other. The libraries produced highly reproducible GC coverage profiles, yielding a Pearson correlation coefficient of 97.26% ± 1.07%.