With our CleanPlex UMI technoloy, one can detect variations down to 0.1% allele frequency. We recommend using 30-50 ng of genomic DNA input for somatic variant detection at 0.1%, or 20-30 ng for 0.25%.  More input (50-80 ng input) may be necessary to improve variant calling when DNA quality is uncertain (such as DNA from FFPE tissues and liquid biopsy).