What is the recommended minimum read coverage for Cleanplex NGS panels?

For Genotyping applications, minimum read coverage per amplicon is 20X.

However, we suggest starting with 1000 PE reads/amplicon/sample for the first sequencing run to account for any potential off target, non-mapping, or poor quality sequencing runs. For subsequent sequencing runs, the allocated PE reads/amplicons can be significantly reduced based on the panel’s performance and sequencing quality.

For Somatic mutation applications, minimum read coverage per amplicon is 500X for 1% allele frequency detection, and 200x for 5% allele frequency detection.

We suggest starting with 2500 PE reads/amplicon/sample for 1% allele frequency detection for the first sequencing run. For follow up sequencing runs, The reads/amplicons allocated can be significantly reduced based on the panel’s performance and sequencing quality.