What is the optimal range of library yields for sequencing?

CleanPlex® workflow generates a single peak of library at 20,000 – 100,000 pM when measured with Agilent™ 2100 Bioanalyzer instrument and Agilent™ high sensitivity DNA reagents, or 3.5 – 20 ng/µl when measured by Qubit™ dsDNA HS Assay Kit, depending on each specific panel. Concentrations higher or lower than this range of yields may lead to lower uniformity due to uneven amplification of target regions.

2018-03-27T15:22:40+00:00 December 28th, 2016|Comments Off on What is the optimal range of library yields for sequencing?