CleanPlex® SARS-CoV-2 Emerging Variants Panel Add-on for robust whole-genome coverage
The SARS-CoV-2 Emerging Variant Panel Add-on is designed to be used with the existing CleanPlex SARS-CoV-2 products to maintain high coverage for variant calling and identification as the virus evolves. Follow the links here to learn more about the Original CleanPlex SARS-CoV-2 and FLEX Panels.
The new variants of concern, such as the UK (B.1.1.7) and South Africa (B.1.351) lineages, contain mutations in the receptor-binding domain (RBD) of the spike protein, which have been shown to significantly increase infection rates and can negatively impact the efficacy of the recently developed vaccines and current COVID-19 qPCR mass testing methods. Thus, a robust detection method and continued screening and surveillance are more critical than ever in our continued global efforts to control this pandemic.
The Add-on primers were generated by cross-referencing the FLEX panel design with selected emerging SARS-CoV-2 variants for uniform coverage and confident identification of their defining mutations.
Highlights of the FLEX panel
- Robust Coverage
Added primers for enhanced coverage of newly discovered UK and South African variants.
- Additional Confidence in Variant Calling
Optimized for identifying existing and new mutations for surveillance and tracking.
- Easy Spike-in Workflow
Directly add to existing CleanPlex SARS-CoV-2 and FLEX panels for ease of use and flexible future updates.
- Robust Coverage
The CleanPlex SARS-CoV-2 Panels contains the CleanPlex Multiplex PCR Primers and CleanPlex Targeted Library Kit with RT components. CleanPlex Indexed PCR Primers for sample pooling and CleanMag® Magnetic Beads for bead purification and size selection are ordered separately to complete the workflow from input RNA to sequencing-ready NGS libraries.
For a complete solution, please use the following indexed PCR primers and magnetic beads SKUs.
|Pack Size (Reactions)||Main Panel & Kits Required with Add-on Primers||SKUs|
|96/384||CleanPlex® SARS-CoV-2 FLEX Panel||918014/ 918015|
|96/384||CleanPlex® SARS-CoV-2 Panel||918011/ 918012|
|Pack Size (Reactions)||SKUs for Indexed PCR Primers||SKUs for Magnetic Beads|
|96||1 x SKU#716006 – CleanPlex® Dual-Indexed PCR Primers for Illumina® Set A (12 x 8 indexes, 96 reactions)||1 x SKU#718002 – CleanMag Magnetic Beads (20 mL, ~100 reactions for CleanPlex 2-pool panels)|
|384||1 x SKU#716017 – CleanPlex® Dual-Indexed PCR Primers for Illumina® Set A (12 x 8 indexes, 384 reactions)||1 x SKU#718003 – CleanMag Magnetic Beads (60 mL, ~300 reactions for CleanPlex 2-pool panels)|
|4 X 96||1 x Each of SKU#716037 to 716040 – CleanPlex® Unique Dual-Indexed PCR Primers for Illumina® Set C, D, E, F 96-well Plated (96 reactions)|
*Note: Use 8 sets of 384 on NovaSeq dual S4 flow cell sequencing for maximum 3072 sample multiplexing.
|1 x SKU#718003 – CleanMag Magnetic Beads (60 mL, ~300 reactions for CleanPlex 2-pool panels)|
Store at -20 °C.
For Research Use Only. Not for use in diagnostic procedures.