What is the recommended minimum read coverage?

For Genotyping applications, minimum read coverage per amplicon is 20X

Although, we suggest starting with 500 reads/amplicon/sample for the first sequencing run. For following sequencing runs, the reads/amplicons can be significantly reduced based on the panel’s performance and sequencing quality.

For Somatic mutation applications, minimum read coverage is 500X for 1% allele frequency detection, and 200x for 5% allele frequency detection.

we suggest starting with 1000 reads/amplicon/sample for 1% allele frequency detection for the first sequencing run. For following sequencing runs, The reads/amplicons allocated can be significantly reduced based on the panel’s performance and sequencing quality.

2018-09-12T13:53:47+00:00 September 12th, 2018|Comments Off on What is the recommended minimum read coverage?