Amplicon Sequencing for CRISPR Gene Editing QC

Amplicon Sequencing for CRISPR Gene Editing QC

Quality control (QC) is an important part of CRISPR gene editing to ensure the accuracy and efficiency of your edits. Amplicon sequencing has emerged as a powerful tool for this purpose. Here, we’ll explore how targeted amplicon sequencing can be used to monitor and validate CRISPR gene editing, providing you with reliable and precise QC measures.

CRISPR Gene Editing QC Workflow

To ensure the accuracy and efficiency of CRISPR gene editing, a robust QC workflow is essential. This workflow involves several steps designed to provide comprehensive insights into the gene editing process using targeted amplicon sequencing:

1. Design Primer Pairs: The first step involves designing primer pairs specific to the target site. These primers will amplify the regions where edits are expected.
2. PCR Amplification: Using PCR amplification, the target regions are enriched, preparing them for sequencing.
3. Sequencing and Data Analysis: The amplified regions are then sequenced using NGS platforms. Detailed sequence analysis and data analysis follow, providing insights into both on-target modifications and potential off-target sites.

Addressing Off-Target Effects

One of the primary concerns in CRISPR gene editing is the occurrence of off-target effects. Targeted sequencing methods such as amplicon sequencing help identify and quantify these off-target sites, ensuring that any unintended edits are detected. This capability validates the gene editing efficiency and minimizes potential off-target effects.

CleanPlex® Technology For CRISPR QC

At Paragon Genomics, our CleanPlex® technology exemplifies the power of targeted amplicon sequencing for CRISPR gene editing QC. CleanPlex® panels provide:

• High Sensitivity and Specificity: Our technology ensures accurate detection of on-target and off-target edits, even in complex samples.
• Efficient Workflow: The streamlined CleanPlex® workflow allows for rapid preparation and sequencing of samples, saving you time and resources.
• Comprehensive Data: With the ability to target multiple regions simultaneously, CleanPlex® provides a thorough analysis of CRISPR edits.

Using CleanPlex® technology, you can confidently assess the accuracy and efficiency of your CRISPR-Cas9 edits, ensuring that your genome editing projects meet the highest standards of quality and reliability.

CRISPR Gene Editing with Amplicon Sequencing

Amplicon sequencing stands out as a superior method for CRISPR gene editing QC. With its targeted approach, sensitivity, and efficiency, it provides the detailed insights needed to validate your gene edits. Paragon Genomics’ CleanPlex® technology further enhances this process, offering a robust solution for your CRISPR QC needs.

By integrating CleanPlex® into your workflow, you can achieve precise, reliable, and efficient quality control for your CRISPR gene editing projects, advancing your research with confidence.