Product Description
The CleanPlex® Single-Indexed PCR Primers v2 for Ion Torrent™ are high-quality ready-to-use PCR primers for Ion Torrent library construction. They are compatible and designed for use with all CleanPlex NGS Panels to construct targeted libraries for sequencing on Ion Torrent NGS platform. The index sets comprise of primer pools for indexing samples for multiplexing samples per sequencing run. Samples with different indexes can be pooled, sequenced together, then demultiplexed for analysis.
| CleanPlex Single-Indexed PCR Primers v2 for Ion Torrent | ||||
| Description | SKU | Reactions | Available indexes/Kit | Format |
| CleanPlex Single-Indexed PCR Primers for Ion Torrent Set T (8 indexes, 8 reactions) | 716016 | 8 | 8 Index pools (Random selection from Set C-F) | 8 Tubes |
| CleanPlex Plated Single-Indexed PCR Primers v2 for Ion Torrent Set C (96 indexes, 192 reactions) | 716053 | 192 | 96 Index pools | 96 well plates |
| CleanPlex Plated Single-Indexed PCR Primers v2 for Ion Torrent Set D (96 indexes, 192 reactions) | 716054 | 192 | 96 Index pools | 96 well plates |
| CleanPlex Plated Single-Indexed PCR Primers v2 for Ion Torrent Set E (96 indexes, 192 reactions) | 716055 | 192 | 96 Index pools | 96 well plates |
| CleanPlex Plated Single-Indexed PCR Primers v2 for Ion Torrent Set F (96 indexes, 192 reactions) | 716056 | 192 | 96 Index pools | 96 well plates |
Primer Sequences
Each sample is indexed by two pairs of Indexed PCR Primers for sequencing on Ion Torrent platforms. XXXXXXXXXX[XX] denotes the 10-12bp index region of the primer. [GGT]GAT denotes the 3-6bp stuffer signature.
Indexed PCR Primers
5’-CCATCTCATCCCTGCGTGTCTCCGACTCAGXXXXXXXXXX[XX][GGT]GATTTCAGACGTGTGCTCTTCCGATCT-3′
5’-CCATCTCATCCCTGCGTGTCTCCGACTCAGXXXXXXXXXX[XX][GGT]GATCCTACACGACGCTCTTCCGATCT-3′
Universal PCR Primers
5’-CCGCTTTCCTCTCTATGGGCAGTCGGTGATTTCAGACGTGTGCTCTTCCGATCT-3′
5’-CCGCTTTCCTCTCTATGGGCAGTCGGTGATCCTACACGACGCTCTTCCGATCT-3′
Note:
- Please refer to CleanPlex NGS Panel User Guide for instructions on using CleanPlex Single-Indexed PCR Primers for Ion Torrent
- Sets C – F are offered at 96 well plated format.
Storage Temperature:
Store at -20°C
For Research Use Only. Not for use in diagnostic procedures.
