Resources
Understand the important challenges that should be considered when preparing NGS sequencing libraries
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What constitutes a good targeted Next-Generation Sequencing library?
Next-generation sequencing (NGS) continues to revolutionize personalized medicine as NGS technology improves and costs continue to fall, resulting in an increased number of scientific and health-related applications. However, the success of a sequencing project rests on the sample quality and NGS library used to generate the data. Poor quality libraries will produce poor quality or biased sequencing data that can slow the advancement and clinical utility of this great new technology.
What is the difference between hybridization capture and multiplex PCR?
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What would you do with the extra time?
Next- generation sequencing (NGS) technologies have accelerated research efforts especially with multiplex PCR (mPCR). Scientists are capable of utilizing NGS to detect and analyze gene sequences in humans,animals, microorganisms, and plants for species authentication, de novo sequencing, and variant identification. Amplicon- based targeted library preparation methods, utilizing mPCR, are preferred by many as the more time and cost-efficient method over hybrid-capture methods. CleanPlex® technology’s simple workflow produces NGS libraries with low background, which means you only sequence the targets of interest and none of the non-specific by-products.