Product Description
The CleanPlex® Plated Unique Dual-Indexed (UDI) PCR Primers for Illumina® are high-quality ready-to-use PCR primers for Illumina library construction. They are compatible and designed for use with all CleanPlex Panels to construct targeted libraries for sequencing on an Illumina NGS platform. The index set combines uniquely pooled i5 and i7 index primer pools for unique dual multiplexing of samples per sequencing run. Each set of UDI primer pools only contains one instance of each i5 and each i7 to mitigate index hopping concerns on Illumina sequencing platforms with patterned flow-cells. Samples indexed with each i5 and i7 pair can be pooled and sequenced, then demultiplexed for analysis.
Discover how our CleanPlex Unique Dual-Indexed PCR Primers for Illumina can support your work. Contact us and request a trial kit today!
| CleanPlex Unique Dual-Indexed (UDI) PCR Primers for Illumina | ||||
| Description | SKU | Reactions | Available Unique Indexes/Kit | Format |
| CleanPlex® Unique Dual-Indexed PCR Primers for Illumina Set T8 (8 indexes, 8 reactions)† | 716015 (Available upon request) |
8 | 8 Unique Index Pairs | 8 Tubes |
| CleanPlex® Plated Unique Dual-Indexed PCR Primers for Illumina Set D 96-well Plate (96 indexes, 96 reactions) | 716037 | 96 | 96 Unique Index pairs | 96 well plates |
| CleanPlex® Plated Unique Dual-Indexed PCR Primers for Illumina Set D 96-well Plate (96 indexes, 96 reactions) | 716038 | 96 | 96 Unique Index pairs | 96 well plates |
| CleanPlex® Plated Unique Dual-Indexed PCR Primers for Illumina Set E 96-well Plate (96 indexes, 96 reactions) | 716039 | 96 | 96 Unique Index pairs | 96 well plates |
| CleanPlex® Plated Unique Dual-Indexed PCR Primers for Illumina Set F 96-well Plate (96 indexes, 96 reactions) | 716040 | 96 | 96 Unique Index pairs | 96 well plates |
| CleanPlex® Plated Unique Dual-Indexed PCR Primers for Illumina Set C 96-well Plate (96 indexes, 384 reactions) | 716049 | 384 | 96 Unique Index pairs | 96 well plates |
| CleanPlex® Plated Unique Dual-Indexed PCR Primers for Illumina Set D 96-well Plate (96 indexes, 384 reactions) | 716050 | 384 | 96 Unique Index pairs | 96 well plates |
| CleanPlex® Plated Unique Dual-Indexed PCR Primers for Illumina Set E 96-well Plate (96 indexes, 384 reactions) |
716051 | 384 | 96 Unique Index pairs | 96 well plates |
| CleanPlex® Plated Unique Dual-Indexed PCR Primers for Illumina Set F 96-well Plate (96 indexes, 384 reactions) |
716052 | 384 | 96 Unique Index pairs | 96 well plates |
*Sets are mutually exclusive. Can be combined for a total of 384 available UDI options.
† Set T8 contains 8 nucleotide sequences and cannot be sequenced with the plated UDIS, which contain 10 nucleotide sequences.
Primer Sequences:
i5 Indexed Primers
5’-AATGATACGGCGACCACCGAGATCTACACXXXXXXXXXXACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′
i7 Indexed Primers
5’-CAAGCAGAAGACGGCATACGAGATXXXXXXXXXXGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3’
Notes:
- This product listing is for indexes delivered in 96 well plated format.
- Each set of sequences is mutually exclusive and can be sequenced together for a total of 384 sample multiplexing
- This set of 384 UDIs contain 10 nucleotide sequences and cannot be sequenced with 8 nucleotide sequences or other Index offerings that include the UDI sets A and B from Paragon Genomics.
Storage Temperature:
Store at -20°C
For Research Use Only. Not for use in diagnostic procedures.
