CleanPlex Amplicon Sequencing Library Kit Product Image

CleanPlex TP53 Kit

Price range: $336.00 through $11,692.80

The CleanPlex® TP53 Kit is a multiplex PCR-based targeted resequencing assay designed to simplify the evaluation of somatic and germline variants across the TP53 gene.

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Product Description

The CleanPlex TP53 Kit is a multiplex PCR-based targeted resequencing assay designed to simplify the evaluation of somatic and germline variants across the TP53 gene. The panel targets all exonic regions and flanking intronic sequences of TP53. Starting with just 20 ng of DNA, sequencing-ready libraries can be prepared using a streamlined workflow in just 3 hours. The kit is optimized to deliver data with high on-target performance and high coverage uniformity to ensure efficient use of sequencing reads.

Highlights

  • Sensitive Detection
    Detect somatic mutations as low as 1% variant allele frequency using just 20 ng of DNA
  • Fast, Streamlined Workflow
    Generate sequencing-ready libraries in just 3 hours using a rapid, three-step protocol
  • Superb Performance
    Prepare high-quality NGS libraries with excellent on-target performance using CleanPlex Technology to enable efficient use of sequencing reads and reduce costs

The CleanPlex TP53 Kit contains CleanPlex Multiplex PCR Primers and CleanPlex Targeted Library Kit. CleanPlex Indexed PCR Primers and CleanMag® Magnetic Beads are ordered separately to complete the workflow from input DNA to sequencing-ready NGS libraries.

Paragon Genomics has partnered with VarSome Clinical to provide a full solution for fast and accurate variant discovery, annotation, and interpretation of next-generation sequencing data for targeted gene panels. Click here to find out more!

Storage Temperature

Store at -20 °C.

For Research Use Only. Not for use in diagnostic procedures.

Additional Information

Weight 40 g
Dimensions 30 × 50 × 20 cm
Pack Size (Reactions)

8, 16, 48, 96, 384

What is the CleanPlex® amplicon sequencing workflow for a multi-pool panel?

For a panel with two or more primer pools, follow the procedure shown below.

There is little or no library peak when assayed with a fragment analyzer.

Possibilities include:

  • Using incompatible index primers.
  • DNA quantification was inaccurate, especially if using spectrophotometric methods such as nanodrop, OR DNA quality is extremely poor.
  • 30% ethanol instead of 70% ethanol OR using TE/H2O instead of 70% ethanol was used in DNA purification.
  • Forgetting to add magnetic beads for any of the purification steps.
  • Forgetting to add one or both index primers in the 2nd PCR step.
  • Over-digestion, forgetting to add Stop Buffer after digestion, or pausing after digestion step.
  • Weak or incompatible magnetic rack. Do not use racks designed for 1.5 ml tube.

What is the typical yield of a Cleanplex® library?

CleanPlex® workflow generates a single peak of library at approximately 8,000 – 20,000 pM when measured with a fragment analyzer such as Agilent™ 2100 Bioanalyzer instrument and Agilent™ high sensitivity DNA reagents, or approximately 1.5 – 4 ng/µl when measured by Qubit™ dsDNA HS Assay Kit, depending on each specific panel. It is good practice to QC completed library with a fragment analyzer to confirm the quality of the library prior to sequencing. Qubit measurements only give the total yield, and does not indicate if the library was poorly prepared and had resulted in large amount of byproducts.

Concentrations higher or lower than the typical range of yields may lead to lower uniformity due to uneven amplification of target regions. Concentrations much lower in concentration can prove to be difficult to satisfy Illumina’s loading criteria. Please refer to Illumina’s suggestions for minimum loading concentration and volume.

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CleanPlex Amplicon Sequencing Library Kit Product ImageCleanPlex TP53 Kit
Price range: $336.00 through $11,692.80Select options