Technical Note – CleanPlex® Technology: Ultrafast, Simple, and Scalable Targeted Sequencing Solution for All Sequencing Platforms

To overcome shortcomings in existing target enrichment techniques and to bridge the gap between amplicon- and hybrid capture-based methods, scientists at Paragon Genomics have developed the CleanPlex® technology to provide a better tool to prepare NGS libraries for targeted sequencing. This technology is an ultra-high multiplex PCR-based target enrichment method that features a proprietary background cleaning chemistry, which allows tens of thousands of amplicons to be pooled in one reaction to target genomic regions as large as a few megabases in size.