The CleanPlex for MGI Single-Indexed PCR Primers are high-quality ready-to-use PCR primers for DNBSEQ™ library construction. They are compatible and designed for use with all CleanPlex for MGI NGS Panels to construct targeted libraries for sequencing on an MGI NGS platform. The index sets comprise of primer pools that form uniquely indexing samples for multiplexing samples per sequencing run. Samples with using unique indexes can be pooled, sequenced together,  then demultiplexed for analysis. 

Primer Sequences:

Each sample is indexed by a pair of Indexed PCR Primers for sequencing on MGISEQ platforms.
XXXXXXXXXX denotes the index region of the primer.

Universal Primer

5′-Phos-GAACGACATGGCTACGATCCGACTT-3’

Indexed Primers

5′-TGTGAGCCAAGGAGTTGXXXXXXXXXXTTGTCTTCCTAAGACCGCTTGGCCTCCGACTT-3’

Note:

• Please refer to CleanPlex for MGI NGS Panel User Guide for instructions on using CleanPlex for MGI Single-Indexed PCR Primers.
• Use a compatible circularization kit with single indexed primers.
• This plated product contains the same primers as tubed indexes set A- F ( SKU 318001-318012)
• The trial kit is not plated, but includes 8 tubed indexes. 

Storage Temperature:

Store at -20°C

For Research Use Only. Not for use in diagnostic procedures.