CleanPlex® Hyper-IgE Syndrome Panel

The CleanPlex® Hyper-IgE Syndrome Panel is a pre-designed and made-to-order multiplex PCR / amplicon-based targeted sequencing assay for examining the germline variants or mutations across 8 genes associated with Hyper-IgE Syndrome.

Product Description

The CleanPlex® Hyper-IgE Syndrome Panel is a pre-designed and made-to-order multiplex PCR / amplicon-based targeted sequencing (NGS) assay designed to examine the germline variants or mutations across 8 genes associated with Hyper-IgE Syndrome. The panel targets all the exonic regions of those genes and the flanking intronic sequences. Compatible with just 10 ng of DNA, sequencing-ready libraries can be prepared using a streamlined workflow in just 3 hours. The pre-designed panel is optimized in silico to deliver data with high on-target performance and high coverage uniformity to ensure efficient use of sequencing reads.

This product is made to order. Once we receive your order, we will synthesize the panel and the kit will contain CleanPlex Multiplex PCR Primers and CleanPlex Targeted Library Kit. CleanPlex Indexed PCR Primers and CleanMag® Magnetic Beads can be ordered separately to complete the workflow from input DNA to sequencing-ready NGS libraries.

Storage Temperature

Store at -20 °C.

For Research Use Only. Not for use in diagnostic procedures.

Gene List:
 SPINK5, STAT3, TYK2, DOCK8, PGM3, SPINK5, STAT3, TYK2

References:
Stray-Pedersen A, et al. PGM3 mutations cause a congenital disorder of glycosylation with severe immunodeficiency and skeletal dysplasia. Am J Hum Genet. 2014 Jul 3;95(1):96-107.

Sassi A, et al. Hypomorphic homozygous mutations in phosphoglucomutase 3 (PGM3) impair immunity and increase serum IgE levels. J Allergy Clin Immunol. 2014 May;133(5):1410-9, 1419.e1-13.

Engelhardt KR, et al.The extended clinical phenotype of 64 patients with dedicator of cytokinesis 8 deficiency. J Allergy Clin Immunol. 2015 Aug;136(2):402-12.

Additional Information

Weight40 g
Dimensions10 × 7 × 5 cm
Pack Size (Reactions)

384

CleanPlex Amplicon Sequencing Library Kit Product ImageCleanPlex® Hyper-IgE Syndrome Panel