CleanPlex® Hereditary Hemophagocytic Lymphohistiocytosis (HLH) Panel

The CleanPlex® Hereditary Hemophagocytic Lymphohistiocytosis (HLH) Panel is a pre-designed and made-to-order multiplex PCR / amplicon-based targeted sequencing assay for examining the germline variants or mutations across 32 genes associated with Hereditary Hemophagocytic Lymphohistiocytosis (HLH).

Product Description

The CleanPlex® Hereditary Hemophagocytic Lymphohistiocytosis (HLH) Panel is a pre-designed and made-to-order multiplex PCR / amplicon-based targeted sequencing (NGS) assay designed to examine the germline variants or mutations across 32 genes associated with Hereditary Hemophagocytic Lymphohistiocytosis (HLH). The panel targets all the exonic regions of those genes and the flanking intronic sequences. Compatible with just 10 ng of DNA, sequencing-ready libraries can be prepared using a streamlined workflow in just 3 hours. The pre-designed panel is optimized in silico to deliver data with high on-target performance and high coverage uniformity to ensure efficient use of sequencing reads.

This product is made to order. Once we receive your order, we will synthesize the panel and the kit will contain CleanPlex Multiplex PCR Primers and CleanPlex Targeted Library Kit. CleanPlex Indexed PCR Primers and CleanMag® Magnetic Beads can be ordered separately to complete the workflow from input DNA to sequencing-ready NGS libraries.

Storage Temperature

Store at -20 °C.

For Research Use Only. Not for use in diagnostic procedures.

Gene List:
ADA, AP3B1, BLOC1S6, BTK, CD27, CD70, FADD, FAS, FASLG, GATA2, IL2RA, IL2RG, ITK, LYST, MAGT1, MEFV, MVK, MYO5A, NLRC4, NLRP3, PNP, PRF1, RAB27A, RECQL4, SH2D1A, SLC7A7, STX11, STXBP2, TNFRSF1A, UNC13D, WAS, XIAP

References:
Badolato R, et al. Exome sequencing reveals a pallidin mutation in a Hermansky-Pudlak-like primary immunodeficiency syndrome. Blood. 2012 Mar 29;119(13):3185-7.

Goudy K, et al. Human IL2RA null mutation mediates immunodeficiency with lymphoproliferation and autoimmunity. Clin Immunol. 2013 Mar;146(3):248-61.

Izawa K, et al. Inherited CD70 deficiency in humans reveals a critical role for the CD70-CD27 pathway in immunity to Epstein-Barr virus infection. J Exp Med. 2017 Jan;214(1):73-89.

Salzer E, et al. Combined immunodeficiency with life-threatening EBV-associated lymphoproliferative disorder in patients lacking functional CD27. Haematologica. 2013 Mar;98(3):473-8.

Additional Information

Weight40 g
Dimensions10 x 7 x 5 cm
Pack Size (Reactions)

384