Why are the short amplicons under-represented in my target enrichment library?
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Why are the long amplicons under-represented in my target enrichment library?
CleanPlex® supports the amplification of up to 300 bp insert length. Please make sure that the correct read length is chosen during sequencing.
Why are the long amplicons under-represented in my target enrichment library? Read More »
What are the AT and GC biases of CleanPlex® target enrichment libraries?
The observed AT and GC dropouts are below 3% in CleanPlex® target enrichment libraries. Below is a typical graph of Coverage Depth vs. GC Content of a library made with CleanPlex® OncoZoom Panel.
What are the AT and GC biases of CleanPlex® target enrichment libraries? Read More »
What is the uniformity of a typical CleanPlex® target enrichment library?
The uniformity ( ≥ 0.2X mean depth) of most CleanPlex® libraries are larger than 95%. For example, the observed uniformities (at ≥ 0.2X mean depth) of CleanPlex® OncoZoom Panel are greater than 99% for both NA12878 (a genomic DNA standard) and Horizon Discovery’s HD780 (a cfDNA standard) (see the graph below).
What is the uniformity of a typical CleanPlex® target enrichment library? Read More »