Archives: Global FAQs

Ready-to-use panels are initiated and designed by the Paragon Genomics R&D team that aim to provide the best solutions to specific research areas and/or clinical needs. The contents of these panels, as well as the performance, are internally validated to ensure that it passes our high standards. Custom panels are designed to satisfy the need …

What are the differences between a ready-to-use panel and a custom panel? Read More »

To submit a custom design request, you will need the following: 1. A Paragon Genomics customer account. Register here. 2. If you are targeting genomes other than human and mouse, be ready to provide the reference genome for design upon request; 3. If your targets are not human genes, please provide the target region in …

How do I submit a custom panel design request? Read More »

Short answer is no. We have designed panels that contain as little as 2 amplicons and we can also offer single-amplicon options. However, it is recommended that a panel contains at least 10 amplicons per pool, to best utilize our multiplex capabilities, and to achieve the best sequencing results from our technology.

We design panels based on our ability to multiplex tens of thousands of amplifications in a single tube. Amplicons that cannot be multiplexed in the same tube are put in separate tubes. Each separate tube of mixture of primers is called a primer pool. In common cases, hotspot targets that are far apart from one …

What are primer pools? How many pools do I need? Read More »

It depends on the difficulty of the design. We have a robust assay design algorithm that handles most designs relatively quickly. One can expect between 3 days to 2 weeks turnaround time (TAT) starting from the moment our design team start working on the requests. The following are the most common reasons for potentially longer …

How soon will I get my custom designs after submission? Read More »

For Genotyping applications, minimum read coverage per amplicon is 20X Although, we suggest starting with 500 reads/amplicon/sample for the first sequencing run. For following sequencing runs, the reads/amplicons can be significantly reduced based on the panel’s performance and sequencing quality. For Somatic mutation applications, minimum read coverage is 500X for 1% allele frequency detection, and …

What is the recommended minimum read coverage? Read More »

We can design targeted panels for any target with a reference sequence.  Some examples of target species include humans, animals, plants, insects, bacteria, and viruses. Our sample types include, but are not limited to cfDNA, FFPE samples, blood samples, and tissue samples. For bacterial panels, a minimum 7 amplicons is required. We can build 7 …

What types of custom panels can you design? Read More »

Using the Cleanplex system, SNV and CNV can be detected. Fusion detection on the other hand is not compatible with our chemistry.

Support documents In addition to the User Guide, please download and review the Quick Guide from the Product Document page for details on some often overlooked but critical steps. You can also refer to the Videos page for the series of short videos that demonstrate the Cleanplex protocol step by step. DNA Quality and Quantification The quality …

What are the key steps in the Cleanplex workflow that I should know prior to starting? Read More »

Yes, please contact [email protected] for a quote.