CleanPlex® Autoimmune Lymphoproliferative Disorders (ALPS) Panel

The CleanPlex® Autoimmune Lymphoproliferative Disorders (ALPS) Panel is a pre-designed and made-to-order multiplex PCR / amplicon-based targeted sequencing assay for examining the germline variants or mutations across 17 genes associated with Autoimmune Lymphoproliferative Disorders (ALPS).

Product Description

The CleanPlex® Autoimmune Lymphoproliferative Disorders (ALPS) Panel is a pre-designed and made-to-order multiplex PCR / amplicon-based targeted sequencing (NGS) assay designed to examine the germline variants or mutations across 17 genes associated with Autoimmune Lymphoproliferative Disorders (ALPS). The panel targets all the exonic regions of those genes and the flanking intronic sequences. Compatible with just 10 ng of DNA, sequencing-ready libraries can be prepared using a streamlined workflow in just 3 hours. The pre-designed panel is optimized in silico to deliver data with high on-target performance and high coverage uniformity to ensure efficient use of sequencing reads.

This product is made to order. Once we receive your order, we will synthesize the panel and the kit will contain CleanPlex Multiplex PCR Primers and CleanPlex Targeted Library Kit. CleanPlex Indexed PCR Primers and CleanMag® Magnetic Beads can be ordered separately to complete the workflow from input DNA to sequencing-ready NGS libraries.

Storage Temperature

Store at -20 °C.

For Research Use Only. Not for use in diagnostic procedures.

Gene List:
CASP10, CASP8, CTLA4, FADD, FAS, FASLG, ITK, KRAS, LRBA, MAGT1, NRAS, PIK3CD, PRKCD, SH2D1A, STAT3, TNFRSF6, XIAP

References:
Takagi M, et al. Haploinsufficiency of TNFAIP3 (A20) by germline mutation is involved in autoimmune lymphoproliferative syndrome. Journal of Allergy and Clinical Immunology. 2017 June; 139(6):1914-1922.

Holzelova E, et al. Autoimmune Lymphoproliferative Syndrome with Somatic Fas Mutations. N Engl J Med 2004; 351:1409-1418.

Zhu S, et al. Genetic alterations in caspase-10 may be causative or protective in autoimmune lymphoproliferative syndrome. (2006) Hum Genet. 119: 284–294.

Additional Information

Weight40 g
Dimensions10 x 7 x 5 cm
Pack Size (Reactions)

384