Product Description
The CleanPlex® Autism Spectrum Disorders Panel is a pre-designed and made-to-order multiplex PCR / amplicon-based targeted sequencing (NGS) assay designed to examine the germline variants or mutations across 79 genes associated with Autism Spectrum Disorders. The panel targets all the exonic regions of those genes and the flanking intronic sequences. Compatible with just 10 ng of DNA, sequencing-ready libraries can be prepared using a streamlined workflow in just 3 hours. The pre-designed panel is optimized in silico to deliver data with high on-target performance and high coverage uniformity to ensure efficient use of sequencing reads.
This product is made to order. Once we receive your order, we will synthesize the panel and the kit will contain CleanPlex Multiplex PCR Primers and CleanPlex Targeted Library Kit. CleanPlex Indexed PCR Primers and CleanMag® Magnetic Beads can be ordered separately to complete the workflow from input DNA to sequencing-ready NGS libraries.
Storage Temperature
Store at -20 °C.
For Research Use Only. Not for use in diagnostic procedures.
Gene List:
ADNP, ADSL, ALDH5A1, ANKRD11, ARID1B, ARX, AUTS2, BCL11A, BRAF, CACNA1C, CC2D1A, CDKL5, CHD2, CHD7, CHD8, CNTN6, CNTNAP2, CREBBP, CSNK2A1, CTNND2, DHCR7, DYRK1A, EHMT1, FMR1, FOLR1, FOXG1, FOXP1, GABRB3, GAMT, GRIN2B, GRIP1, HDAC8, HOXA1, HPRT1, KATNAL2, KMT5B, MAGEL2, MBD5, MECP2, MED12, MEF2C, MID1, NHS, NIPBL, NLGN3, NLGN4X, NRXN1, NSD1, PCDH19, POGZ, PQBP1, PTCHD1, PTEN, PTPN11, RAB39B, RAD21, RAI1, RELN, RPL10, SCN1A, SCN2A, SHANK2, SHANK3, SLC6A1, SLC6A8, SLC9A6, SMC1A, SMC3, SPAST, SYNGAP1, TBR1, TCF20, TCF4, TSC1, TSC2, UBE3A, UPF3B, VPS13B, ZEB2
References:
Lauritsen et al. Effects of familial risk factors and place of birth on the risk of autism: a nationwide register-based study. J Child Psychol Psychiatry. 2005;46:963–971.
Saunders et al. Comprehensive ZEB2 gene analysis for Mowat-Wilson syndrome in a North American cohort: a suggested approach to molecular diagnostics. Am J Med Genet A. 2009;149A:2527–31.
Shen et al. Clinical Genetic Testing for Patients With Autism Spectrum Disorders. Pediatrics. 2010 Apr; 125(4): e727–e735.
Fernell et al. Early diagnosis of autism and impact on prognosis: a narrative review. Clin Epidemiol. 2013; 5: 33–43.
Zoghbi HY, Bear MF. Synaptic Dysfunction in Neurodevelopmental Disorders Associated with Autism and Intellectual Disabilities. Cold Spring Harb Perspect Biol. 2012 Mar; 4(3): a009886.
