Why Choose Our Amplicon Based NGS Library Prep Kit for CRISPR QC?
NGS is a powerful tool for validating CRISPR-Cas9 knockouts. Our streamlined solution offers unmatched efficiency and accuracy for your CRISPR QC needs.
- Fast Workflow – Library prep in just 3 hours.
- Simple Protocol – Streamlined, easy-to-use steps.
- Cost-Effective – Reduce sequencing costs with optimized efficiency.
- High Accuracy – Reliable results with low error rates.
- Flexible Applications – Works for CRISPR QC, Genotyping, Microbiome, etc.
| Paragon Genomics CleanPlex® | Competitor T | Competitor A | |
|---|---|---|---|
| Enrichment Type | Multiplex PCR | Multiplex PCR | Hybrid Capture |
| Uniformity | High | Low | High |
| Min. input required | 6 pg (1 cell only) | 1 ng | >20 ng |
| Max. level of multiplexing | >20,000 amplicons | 12,000 amplicons | Whole Exome |
| Single tube workflow? | Yes | No | No |
| Assay time | 3 hours | 5 7 hours | > 1 day |
DNA is extracted from CRISPR-edited Samples, Genomic DNA from Blood or Saliva, Leaf Tissue, Seeds, Animal Tissue or Hair Follicles.
1
Multiplex PCR. Targets are amplified in a multiplex PCR reaction, followed by magnetic bead purification.
2
Background Cleaning. Primer-dimer, non-specific PCR products, and other contaminants are biochemically removed, followed by purification.
3
Indexing PCR. Sequencer-specific adapter sequences and indices are added by PCR, followed by purification.
Final libraries are pooled for sequencing.
Applications
- Off-Target Analysis
- Crop & Trait Engineering
- On-Target Indel Profiling
- Gene Therapy Research
- Genetic Disease Testing
- Cell Line Development
- Infectious Disease Detection
- CRISPR Gene Editing Verification
- KO/KI Confirmation