CleanPlex® Single-Indexed PCR Primers for Ion Torrent™

$304.00$672.00

The CleanPlex® Single-Indexed PCR Primers for Ion Torrent™ are high-quality ready-to-use PCR primers for Ion Torrent library construction. They are compatible and designed for use with all CleanPlex NGS Panels to constructed targeted libraries for sequencing on an Ion Torrent NGS platform.

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Product Description

The CleanPlex® Single-Indexed PCR Primers for Ion Torrent™ are high-quality ready-to-use PCR primers for Ion Torrent library construction. They are compatible and designed for use with all CleanPlex NGS Panels to construct targeted libraries for sequencing on Ion Torrent NGS platform. The index sets comprise of primer pools for indexing samples for multiplexing samples per sequencing run. Samples with different indexes can be pooled, sequenced together,  then demultiplexed for analysis. 

CleanPlex Single-Indexed PCR Primers for Ion Torrent
DescriptionSKUReactionsAvailable indexes/KitFormat
CleanPlex® Single-Indexed PCR Primers for Ion Torrent™ Set T
(8 indexes, 8 reactions)
71601688 Index pools
(Random selection from Set A-F)
8 Tubes
CleanPlex® Single-Indexed PCR Primers for Ion Torrent™ Set A*
(16 indexes, 32 reactions)
7160073216 Index pools16 Tubes
CleanPlex® Single-Indexed PCR Primers for Ion Torrent™ Set A
(16 indexes, 96 reactions)
7160089616 Index pools16 Tubes
CleanPlex® Single-Indexed PCR Primers for Ion Torrent™ Set B*
(16 indexes, 32 reactions)
7160093216 Index pools16 Tubes
CleanPlex® Single-Indexed PCR Primers for Ion Torrent™ Set B
(16 indexes, 96 reactions)
7160109616 Index pools16 Tubes
CleanPlex® Single-Indexed PCR Primers for Ion Torrent™ Set C**
(96 indexes, 192 reactions)
716025 (available upon request)19296 Index pools96 well plates
CleanPlex® Single-Indexed PCR Primers for Ion Torrent™ Set D **
(96 indexes, 192 reactions)
716026
(available upon request)
19296 Index pools96 well plates
CleanPlex® Single-Indexed PCR Primers for Ion Torrent™ Set E **
(96 indexes, 192 reactions)
716027
(available upon request)
19296 Index pools96 well plates
CleanPlex® Single-Indexed PCR Primers for Ion Torrent™ Set F **
(96 indexes, 192 reactions)
716028
(available upon request)
19296 Index pools96 well plates

*Set A and Set B are mutually exclusive. Combined forms 32 indexing options.
**Sets C-F includes Sets A and B indexes, but are unique among each other. These 4 sets of 96 together form 384 indexing options.

Primer Sequences

Each sample is indexed by two pairs of Indexed PCR Primers for sequencing on Ion Torrent platforms. XXXXXXXXX denotes the index region of the primer.

Indexed PCR Primers

5’-CCATCTCATCCCTGCGTGTCTCCGACTCAGXXXXXXXXXCGATTTCAGACGTGTGCTCTTCCGATCT-3′

5’-CCATCTCATCCCTGCGTGTCTCCGACTCAGXXXXXXXXXCGATCCTACACGACGCTCTTCCGATCT-3′

Universal PCR Primers

5’-CCGCTTTCCTCTCTATGGGCAGTCGGTGATTTCAGACGTGTGCTCTTCCGATCT-3′

5’-CCGCTTTCCTCTCTATGGGCAGTCGGTGATCCTACACGACGCTCTTCCGATCT-3′

Note:

  • Please refer to CleanPlex NGS Panel User Guide for instructions on using CleanPlex Single-Indexed PCR Primers for Ion Torrent
  • This product listing is for indexes delivered in single tube format. Sets C – F are offered at 96 well plated format and are available upon request. 
  • Set A and Set B index sequences are mutually exclusive and can be sequenced together for a total of 32 sample multiplexing. Sets C contains indexes from Sets A and B, but are mutually exclusive from sets D, E, and F. Together Paragon offers 384 indexing options for Ion Torrent. 

Storage Temperature:

Store at -20°C

For Research Use Only. Not for use in diagnostic procedures.

Product Documents

Additional Information

WeightN/A
Pack Size (Reactions)

Set A 32, Set A 96, Set B 32, Set B 96

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