Does the digestion step work at other temperatures than 37°C?

Digestion may work at room temperature (25°C) for certain panels with ≤ 220 primer pairs. However, other similar or larger panels may experience incomplete digestion of non-specific amplification products at room temperature. This may cause under-amplification of the library during the 2nd PCR step.

2018-03-27T15:13:29+00:00 December 28th, 2016|Comments Off on Does the digestion step work at other temperatures than 37°C?